- ¥118
- 江蓝纯
- D6581
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
江蓝纯
- 规格：
1000U
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D6581 | PvuII | 1000U | 118.00元 |
PvuII内切酶为进口分装，基本信息如下：
| 识别序列 | 缓冲液兼容性(%) | 酶切温度 | 失活条件 | 甲基化干扰？ | |||||
| CAG^CTG GTC^GAC |
1X B | 1X G | 1X O | 1X R | 1X Y | 2X Y | 37℃ | 80℃ 20min |
基本无干扰** |
| 50-100* | 100 | 20-50 | 50-100 | 20-50* | 20-50* | ||||
*，Star activity，当酶量5倍或以上过量时会产生星号活性，即产生非特异性酶活性。
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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
Mesoplasma florum:Transposome construction
Transposome DNA construction from plasmid cutting Cut the transposome out of the containing plasmid with PvuII enzyme, which cuts at the correct location at the mosaic end. Cut also with an enzyme which produces shorter
Quantification of mRNA Levels Using Ribonuclease Protection Assay
ends (shown by the gray color). ( C ) To prepare synthetic NT-3 mRNA, the plasmid is cut with PvuII , which cuts twice in the plasmid backbone. The presence of the vector-derived PvuII fragment will not interfere
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