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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pcDNA3.1/Myc-His C
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pcDNA3.1/Myc-His C
pcDNA3.1/Myc-HisC载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His C , pcDNA3.1/Myc-HisC |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5490 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: | |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pcDNA3.1/Myc-HisC载体质粒图谱和多克隆位点信息
pcDNA3.1/Myc-HisC载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-HisC载体序列
LOCUS pcDNA3.1(+)/myc-HisC 5490 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5490
/invitrogen="128"
/vntifkey="98"
CDS 1038..1055
/invitrogen="1010000"
/vntifkey="4"
/label=6xHis
CDS complement(4494..5354)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1084..1308
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1078..1095)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5355..5453)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
CDS 993..1022
/invitrogen="1090000"
/vntifkey="4"
/label=c-myc\epitope
rep_origin 1354..1782
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 902..995
/invitrogen="1470000"
/vntifkey="21"
/label=MCS
CDS 2193..2987
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3676..4349)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1787..2157
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3163..3293
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
BASE COUNT 1273 a 1427 c 1397 g 1393 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
961 gcacagtggc ggccgctcga ggtcacccat tcgaacaaaa actcatctca gaagaggatc
1021 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
1081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
1141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
1201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
1261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
1321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
1381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
1441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
1501 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
1561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
1621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
1681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
1741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
1801 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
1861 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
1921 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
1981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
2041 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
2101 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
2161 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
2221 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
2281 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
2341 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
2401 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
2461 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
2521 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
2581 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
2641 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
2701 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
2761 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
2821 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
2881 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
2941 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
3001 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
3061 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
3121 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
3181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
3241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
3301 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
3421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
3481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
3541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
3901 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
4381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
4441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
4501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
4561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
4621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
4681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
4741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
4801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
4861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
4921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
4981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
5281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
5341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
5401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
5461 acatttcccc gaaaagtgcc acctgacgtc
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pcDNA3.1/Myc-His C
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