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pGL4.72[hRlucCP]
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pGL4.72 ; pGL4.72[hRlucCP] |
质粒类型: | 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | 无 |
载体大小: | 3704 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | hRlucCP |
载体抗性: | 氨苄青霉素 |
筛选标记: | 无 |
克隆菌株: | TOP10等常规菌株 |
宿主细胞(系): | HEK293等 |
备注: | pGL4.72[hRlucCP]载体是信号通路报告载体;无启动子;含海肾 荧光素酶报告基因hRluc。 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pGL4.72载体简介The pGL4.72[hRlucCP] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.72[hRlucCP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
LOCUS AY738228 3704 bp DNA circular SYN 08-NOV-2004DEFINITION Renilla luciferase reporter vector pGL4.72[hRlucCP], complete sequence.ACCESSION AY738228VERSION AY738228.1 GI:55535636KEYWORDS .SOURCE Renilla luciferase reporter vector pGL4.72[hRlucCP] ORGANISM Renilla luciferase reporter vector pGL4.72[hRlucCP] other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 3704) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega Corporation JOURNAL UnpublishedREFERENCE 2 (bases 1 to 3704) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE Direct Submission JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USAFEATURES Location/Qualifiers source 1..3704 /organism="Renilla luciferase reporter vector pGL4.72[hRlucCP]" /mol_type="other DNA" /db_xref="taxon:296665" misc_feature 1..70 /note="multiple cloning site region" CDS 100..1212 /codon_start=1 /transl_table=11 /product="luciferase hRlucCP" /protein_id="AAV52881.1" /db_xref="GI:55535637" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV" misc_feature 1249..1470 /note="SV40 late poly(A) region" primer_bind 1538..1557 /note="reporter vector primer 4 (RVPrimer4)" rep_origin 1795 /note="ColE1-derived" gene complement(2586..3446) /gene="ampR" CDS complement(2586..3446) /gene="ampR" /note="AmpR" /codon_start=1 /transl_table=11 /product="synthetic beta-lactamase" /protein_id="AAV52882.1" /db_xref="GI:55535638" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3551..3704 /regulatory_class="polyA_signal_sequence" /note="synthetic; transcriptional pause site" primer_bind complement(3653..3672) /note="reporter vector primer 3 (RVPrimer3)"ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac 121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg 181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg 241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc 301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc 361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg 421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt 481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg 541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag 601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca 661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag 721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag 781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc 841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc 901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc 961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg 1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc 1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc 1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg 1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa 1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc 1501 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc 1561 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc 1621 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 1681 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 1741 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 2041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 2101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 2161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 2221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 2281 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 2341 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 2401 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 2461 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 2521 aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc 2581 agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg 2641 tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca 2701 ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca 2761 atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc 2821 atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc 2881 cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct 2941 tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga 3001 aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg 3061 tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc 3121 ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca 3181 agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa 3241 gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg 3301 agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc 3361 accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt 3421 gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat 3481 cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg 3541 gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga 3601 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa 3661 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctct
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