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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
Cloud-Clone
- 英文名:
Active Cold Inducible RNA Binding Protein (CIRBP)
- 规格:
50ug
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文献和实验Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
volume so there is 12.5ul of each lysate per binding assay. Add 12.5ul of each protein lysate to an eppendorf and mix by pipetting. Allow proteins to associate for 30 minutes at 4°C. Add 200ul of cold immunoprecipitation buffer. Suitable buffers include
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
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