- ¥800 - 1200
- Ybscience
- 中国/美国
- YB-0104
- 2025年07月10日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-26b(+)
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pET-26b(+)
pet26b载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 别名: | pET26b, pet-26b(+), pET 26b(+) |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5360bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | C-His |
| 载体抗性: | 卡那(Kanamycin) |
| 备注: | 含有pelB信号肽，蛋白表达时，能够将目的基因定位于细胞周质。 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pet26b载体质粒图谱和多克隆位点信息
| Feature Name | Start | End |
|---|---|---|
| T7_terminator | 129 | 1 |
| T7_Terminal_primer | 69 | 87 |
| T7_transl_en_RBS | 314 | 298 |
| lacO | 359 | 332 |
| T7_promoter | 377 | 359 |
| tet (300 - 563) | 409 | 672 |
| pBRrevBam_primer | 480 | 461 |
| lacI | 755 | 1846 |
| tet (576 - 851) | 1905 | 2180 |
| ROP | 2655 | 2846 |
| pGEX_3_primer | 2862 | 2840 |
| pBR322_origin | 3880 | 3261 |
| KanR2 | 3986 | 4801 |
| ORF | Start | End |
|---|---|---|
| ORF frame 1 | 514 | 1014 |
| ORF frame 2 | 887 | 1846 |
| ORF frame 2 | 3986 | 4801 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| NcoI | 220 |
| MscI | 225 |
| NdeI | 288 |
| XbaI | 326 |
| BglII | 392 |
| BclI | 1128 |
| ApaI | 1325 |
| HpaI | 1620 |
| FspI | 2196 |
| NruI | 4074 |
| ClaI | 4108 |
| SmaI | 4291 |
| XmaI | 4289 |
pet26b载体简介
The pET-26b(+) vector (Cat. No. 69862-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pet26b载体序列
LOCUS pET-26b(+) 5360 bp DNA circular SYN
DEFINITION pET-26b(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5360
/organism="pET-26b(+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
misc_feature complement(298..314)
/label="T7_transl_en_RBS"
misc_feature complement(332..359)
/label="lacO"
promoter complement(359..377)
/label="T7_promoter"
gene 409..672
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(461..480)
/label="pBRrevBam_primer"
CDS 514..1014
/label="ORF frame 1"
misc_feature 755..1846
/label="lacI"
CDS 887..1846
/label="ORF frame 2"
gene 1905..2180
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
misc_feature 2655..2846
/label="ROP"
misc_feature complement(2840..2862)
/label="pGEX_3_primer"
rep_origin complement(3261..3880)
/label="pBR322_origin"
gene 3986..4801
/label="KanR2"
/gene="KanR2"
CDS 3986..4801
/label="ORF frame 2"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag
4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa
4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
5341 agggcgcgtc ccattcgcca
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
, either alone or in combination with chemo- or radiotherapy at the more advanced stages. However, many patients present with metastatic disease at the time of diagnosis. Both computed tomography (CT) and positron emission tomography (PET) using fluorodeoxyglucose (FDG) play
什么是PET 树脂 ---PET是英文Polyethylene terephthalate的缩写，PET的中文意思是聚对苯二甲酸乙二醇酯。PET是以石油中的对苯二甲酸和乙二醇为原料，在高温高真空条件下进行化学反应而形成的树脂之一。PET瓶具有综合性能优良的特点，在饮料行业被广泛使用。 核医学 ---PET是英文Positron Emission Tomography的缩写，PET的中文意思是正电子发射计算机断层扫描。PET是核医学发展的一项新技术。 PET瓶片－－
PET and PET/CT in the Management of Thyroid Cancer
The introduction of PET(-CT) has brought about a major paradigm shift in the management of thyroid carcinoma, especially from the diagnostic standpoint. From the viewpoint of patient management, the areas where it has made significant impact
技术资料暂无技术资料 索取技术资料
pET-26b(+)
¥800 - 1200
