手机验证
-20℃
6个月
pET-29b(+)
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
别名: | pET29b, pet 29b |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5370 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-S, N-Thrombin, C-His |
载体抗性: | Kanamycin |
表达宿主菌: | BL21(DE3) 、BLR(DE3)、BL21(DE3)pLysS |
备注: | N term thrombin cleavage site; a,b,c vary by MCS; |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
lacI | 217 | 1856 | |
T7_transl_en_RBS | 320 | 304 | |
lacO | 365 | 338 | |
T7_promoter | 383 | 365 | |
tet (300 - 563) | 419 | 682 | |
pBRrevBam_primer | 490 | 471 | |
tet (576 - 851) | 1915 | 2190 | |
ROP | 2665 | 2856 | |
pGEX_3_primer | 2872 | 2850 | |
pBR322_origin | 3890 | 3271 | |
KanR2 | 3996 | 4811 |
ORF | Start | End | |
---|---|---|---|
ORF frame 2 | 296 | 1024 | |
ORF frame 3 | 897 | 1856 | |
ORF frame 3 | 3996 | 4811 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
EcoRV | 206 |
NcoI | 211 |
KpnI | 237 |
BglII | 240 |
BstBI | 267 |
NdeI | 294 |
XbaI | 332 |
BclI | 1138 |
ApaI | 1335 |
HpaI | 1630 |
FspI | 2206 |
NruI | 4084 |
SmaI | 4301 |
XmaI | 4299 |
The pET-29a-c(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No.69337-3).
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