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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
mouse IgG1
- 形态:
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 目录编号:
251034
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
见详细说明信息
- 靶点:
CD62P (HI62P)
- 抗体英文名:
CD62P (HI62P) Antibody
- 抗体名:
CD62P (HI62P) Antibody
- 规格:
2 ml
CD62P (HI62P) Antibody产品详细介绍:
| 产品名称: | CD62P (HI62P) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251034 |
| 规格: | 2 ml |
| 产品描述: | The CD62P (HI62P) antibody recognizes a 140-kDa long single chain type-I transmembrane glycoprotein, P-selectin, also called granule membrane protein-140 (GMP-140) or platelet activation dependent granule-external membrane protein (PADGEM). CD62P antigen is present in the α-granules of platelets and in Weibel-Palade bodies of endothelial cells, and is rapidly mobilized from storage granules to the cell surface after cell activation. CD62P is expressed on the surface of the activated platelets. Interaction of CD62P with PSGL-1 (CD162) mediates tethering and rolling of leukocytes on the surface of activated endothelial cells, the first step in leukocyte extravasation and migration towards sites of inflammation. CD62P also mediates rolling of platelets on endothelial cells and CD62P-mediated interactions involved in platelet-mediated delivery of lymphocytes to high endothelial venules (HEV). Defects in CD62P is a cause of susceptibility to ischemic stroke. |
| 克隆类型: | Mouse Monoclonal Antibody, FITC-conjugated Antibody |
| 亚型: | mouse IgG1 |
| 免疫原: | |
| 适用物种: | H |
| 应用范围: | FC |
| 应用说明: | FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000 |
| 别名: | CD62P; P-selectin; Granule membrane protein 140; GMP-140; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; CD62 antigen-like family member P; SELP |
| 形态: | Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验P- 选择素 (CD62P) 又称PADGEM、LECAM-3或颗粒膜蛋白140(GMP-140)。胞膜外区N端有1个C型凝集素结构域,1个EGF样结构域和9个CCP结构域。P-选择素表达于巨核细胞、活化血小板和活化的内皮细胞。P-选择素贮存于血小板。颗粒和内皮细胞Weibel-Palade小体中,当凝血酶、组胺、PMA或过氧化物活化细胞后,P-选择素从颗粒中迅速与胞膜融合,而表达于细胞膜上,由于膜上的P-选择素很快被内化并在溶酶体中降解,所以P-选择素的膜表达是瞬时
selectin,LAM- 1,LECAM- 1) 主要表达细胞: T,B,M,NK,PMN,Eo[AS] 分子质量(kDa)和结构: gp76(C- lectin SF,含EGF样结构域和2个CCP结构域) 功 能: 粘附CD15s、E- selectin,P- selectin,结合Gly- CAM- 1、CD34、MAd- CAM- 1上的O连接糖 CD62P 常用单克隆抗体或代号: G2,AK- 6;(P- selectin,GMP- 140
. 过碘酸钠标记法IgG量(mg/ml)=(OD280- OD402 ′0.3)′0.94′0.62 标记率= OD403 / OD280 =0.3-0.6,即一个抗体分子上结合1-2个HRP分子.标记率可以大于0.6-0.9。OD403 / OD280 =0.4,约相当于E/P=1.0。 酶标抗体的保存 经鉴定合格的酶标抗体,由于硫化物及叠氮化物等可与酶结合,使HRP失活,因此,不能用硫化物及叠氮化物作为防腐剂,可加入等量的60%甘油,小量分装(0.5ml/支),置4℃~-30℃保存。
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