- ￥3000 - 3999
- Abbiotec
- 美国
- 250286
- 2026年02月02日
- E, WB
- Mouse
- H
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
C3 isolated from human plasma
- 亚型：
Mouse IgG2a/k
- 形态：
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件：
Store at 4°C.
- 克隆性：
单克隆抗体
- 标记物：
见下方详细说明
- 适应物种：
H
- 宿主：
Mouse
- 应用范围：
E, WB
- 浓度：
见详细说明信息
- 靶点：
C3 Complement beta-chain variant (8G4)
- 抗体英文名：
C3 Complement beta-chain variant (8G4) Antibody
- 抗体名：
C3 Complement beta-chain variant (8G4)抗体
- 规格：
0.1 mg
C3 Complement beta-chain variant (8G4) Antibody产品详细介绍：
| 产品名称： | C3 Complement beta-chain variant (8G4) Antibody |
| 说明书： | http://www.abbiotec.com/antibodies/c3-complement-beta-chain-variant-8g4-antibody【参考图片】 |
| 货号： | 250286 |
| 规格： | 0.1 mg |
| 产品描述： | C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of alpha and beta chains (110 kDa and 75 kDa respectively) held together by disulfide bonds. This antibody is specific for an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regularly in the beta-chain of C3F and occasionally in the beta-chain of C3S (F and S meaning fast and slow migration rate, respectively, on agarose gel electrophoresis). The antibody reacts with the 75-kDa beta-chain band of reduced C3 in immunoblotting , and reacts with the 20-kDa and 17-kDa beta-chain fragments produced by cyanogen bromide cleavage. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG2a/k |
| 免疫原： | C3 isolated from human plasma |
| 适用物种： | H |
| 应用范围： | E, WB |
| 应用说明： | Product Cat. No. 250286 reacts strongly with C3. Strong reaction is seen in ELISA with C3 coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in wells coated with a polyclonal antibody against C3. In Western blot, Product Cat. No. 250286 detects the allotypic marker on the beta-chain of C3, closely related but not completely specific to the fast-moving form of C3, C3F. Product Cat. No. 250286 can be cleaved by papain to produce Fab fragments. E: 1:8,000; WB: 1:500 |
| 别名： | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; Complement C3; C3a anaphylatoxin; Complement C3b alpha chain; Complement C3c alpha chain fragment 1; Complement C3dg fragment; C3d fragment; C3g fragment; C3f fragment; C3c alpha chain fragment 2 |
| 形态： | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件： | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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文献和实验The Complement System:An Overview
complex replaces the Cl complex. Regulators act to inhibit either the enzymes of the activation pathways (activated Cl, C3 convertases, C5 convertases) or assembly of the MAC. Table 1 The Component Proteins of the Complement
Immunoaffinity Methods for Purification of Complement Components and Regulators
of membrane regulators of complement and complement receptors (3 ,7 ,8 ). Proteins isolated by immunoaffinity methods, particularly on monoclonal antibody solid phases, are usually of high purity and require either no downstream processing or simple
Purification, Quantification, and Functional Analysis of Complement Factor H
fish. Human FH protein concentration can be quantified by sandwich ELISA. The activity of FH is generally measured by assays which detect the cleavage, by complement factor I, of the complement protein C3b to form iC3b. Cleavage occurs
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