名称:重组人死亡受体6(TNFRSF21)库存 编号:JN0446 规格:10μg|50μg|500μg|1mg 品牌:百奥莱博 英文名:Recombinant Human Death Receptor 6 本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Gln42-Leu350在C端含有His标签。
关于TNFRSF21: Tumor Necrosis Factor Receptor Superfamily Member 21 (TNFRSF21) is a type I transmembrane receptor that includes four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. DR6 may activate NF-kappa-B and JNK to promote apoptosis and T-cell differentiation. In addition, DR6 binds with N-APP, which is released by the deprivation of Trophic-factor. It triggers caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6). DR6 is also expressed on the tumor cell lines and can be induced by TNF-α.
使用方法: 1、Western检测: a. 按照1:1000用相应的Western封闭液稀释抗体。 b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温作用1小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。 c. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。 2、其它实验操作请自行参考相应的方案进行。