Background Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and ε Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Protein such as β-tubulin is frequently used as loading controls for western blots and protein normalization. Description A highly specific and sensitive rabbit polyclonal antibody against β-Tubulin. Source KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human beta-Tubulin. Applications WB 1:1000-2000, ICH 1:500-2000. Optimal dilution has to be determined by the user. Applicable Species mouse, rat, human. Specifications Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography. Storage conditions store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃). Note For research use only, not for use in diagnostic procedures. Data
Western blot analysis of β-Tubulin expression in Rat liver (1), HeLa (2) and NIH/3T3 (3) whole cell lysates