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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Anti-Glutathione-S-transferase (GST) monoclonal antibody
- 供应商:
上海万生昊天生物技术有限公司
- 克隆性:
多克隆
- 规格:
100ul
Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore plays a role in the detoxification of potential alkylating agents. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule, and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.
Description
a mouse monoclonal antibody raised against Glutathione-S-Transferase.
Source
Glutathione-S-Transferase.
Applications
WB 1:1000-2000, ELISA 1:500-2000. Optimal dilution has to be determined by the user.
Applicable Species
GST from Schistosoma japonicum in GST fusion proteins.
Specifications
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.
Storage conditions
store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).
Note
For research use only, not for use in diagnostic procedures.
Data

Western blot analysis of GST-tagged fusion proteins.
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Preparation of Glutathione-S-Transferase (GST) Fusion Proteins Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia, PA 19111 Jason R. Orlinck
Catalytic Function and Expression of Glutathione Transferase Zeta
The zeta class of glutathione S -transferases (GSTZ) is one of the most recently discovered soluble GST classes and has proved to be of considerable interest because of its contribution to the catabolism of phenylalanine and tyrosine
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