Recombinant Mouse IL-7RA (C-Fc

Recombinant Mouse IL-7RA (C-Fc) (CK77)

产品说明(Description)

Recombinant Mouse Interleukin-7 receptor subunit alpha is produced by our Mammalian expression system and the target gene encoding Glu21-Asp239 is expressed with a Fc tag at the C-terminus. 

Accession #: P16872

Known as: Interleukin-7 receptor subunit alpha; Il7r; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CD127

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5.

 

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity-BLI:

 

Loaded Human IL-7-His(Cat#CX47) on HIS1K Biosensor, can bind Mouse IL-7RA-Fc(Cat#CK77) with an affinity constant of 5.91 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY