Recombinant Mouse B7-2/CD86

Recombinant Mouse B7-2/CD86 (CK79)

 

产品说明(Description)

Recombinant Mouse B-lymphocyte activation antigen B7-2 is produced by our Mammalian expression system and the target gene encoding Val26-Glu245 is expressed with a Fc tag at the C-terminus. 

Accession #: P42082

Known as: T-lymphocyte activation antigen CD86; Cd86; Activation B7-2 antigen; Early T-cell costimulatory molecule 1; ETC-1; CD86

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. Mouse CD86 shares 59% and 81% aa sequence identity with human and rat CD86, respectively. It contains 1 Ig-like C2-type domainand 1 Ig-like V-type domain. It is highly expressed on activated antigen presenting cells. CD86 involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY