Recombinant Human CD38

Recombinant Human CD38  (CU65)

产品说明(Description)

Recombinant Human ADP-ribosyl Cyclase/cyclic ADP-ribose Hydrolase 1 is produced by our Mammalian expression system and the target gene encoding Val43-Ile300 is expressed with a 6His tag at the N-terminus. 

Accession #: P28907

Known as: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1 

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制(Quality Control) 

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Anti-Human CD38 mAb(Cat#NC051) at 5μg/ml (100 μl/well) can bind Human CD38-His(Cat#CU65). The ED50 of  Human CD38-His(Cat#CU65) is 62.2 ng/ml.		Loaded Human CD38-His(Cat#CU65) on HIS1K Biosensor, can bind Anti-Human CD38 mAb-Fc(Cat#NC051) with an affinity constant of <10-3 nM as determined in BLI assay.

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景(Background)

CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, is a Signal-anchor for type II membrane protein. CD38 is able to transform NAD+ to  ADP-D-ribose and nicotinamide. It also can transform NADP+ to nicotinate-adenine dinucleotide phosphate and nicotinamide. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY