Recombinant Human Complement F

Recombinant Human Complement Factor MASP3 (C-6His)  (C655)

 

产品说明(Description)

Recombinant Human Complement Factor MASP3 is produced by our Mammalian expression system and the target gene encoding His20-Arg728 is expressed with a 6His tag at the C-terminus. 

Accession #: P48740-2

Known as: Mannan-Binding Lectin Serine Protease 1; Complement Factor MASP-3;Mannose-Binding Lectin-Associated Serine Protease 1; MASP-1; RaRF; Serine Protease 5; MASP1; CRARF; CRARF1; PRSS5

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris,200mM NaCl,10％Glycerol,pH8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

MASP3 is a member of the MASPs involved in mannan-binding lectin (MBL) complement pathway. The MBL pathway is initiated by the binding of MBL to specific carbohydrate structures found on the surface of a variety of microorganisms. Activation of the complement pathway via MBL is initiated by specific MASPs. Three MASPs have been identified and all have domain structures similar to those of C1r and C1s with a heavy chain (chain A) and a light chain (chain B). Chain A is composed of CUB1, EGF, CUB2, CCP1 and CCP2 while chain B corresponds to the catalytic domain found in many serine proteases. MASP1 and MASP3 are two alternatively spliced products of a single gene, which contain the same A chains but entirely different B chains. Distinct MASPs found in different MBL oligomers may have different biological activities. For example, MASP3, found together with MASP2, downregulates the C4 and C2 cleaving activity of MASP2. The protease activity of MASP3 is first revealed here using rhMASP3CD, which is inhibited by serine protease inhibitors such as Ecotin and AEBSF.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Circulating IGF-I and IGFBP5 Levels Control Human Colonic Stem Cell Function and Are Disrupted in Diabetic Enteropathy. Francesca D’Addio. et al. Cell Stem Cell. 2015.

 

INHIBITORS OF IGFBP3 BINDING TO TMEM219 FOR TREATMENT OF INTESTINAL DISEASES[J]. D'addio Francesca. et al. 2020.

 

FOR RESEARCH USE ONLY