Recombinant Human HGF/Hepatocyte Growth Factor (CJ72)
产品说明(Description)
Recombinant Human Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus.
Accession #: P14210
Known as: Hepatocyte growth factor;HPTA;HGF;SF;Scatter factor;Hepatopoietin-A
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Bioactivity: Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
The ED50 for this effect is 0.3-1.5 ng/ml.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
电泳(SDS-PAGE)
参考文献(Reference)
Aging Reprograms the Hematopoietic-Vascular Niche to Impede Regeneration and Promote Fibrosis[J]. Yutian Chen. et al. Cell Metabolism. 2021.
A Nongenetic Proximity-Induced FRET Strategy Based on DNA Tetrahedron for Visualizing the Receptor Dimerization[J]. Yanhua L. et al. Anal. Chem..2020.
FOR RESEARCH USE ONLY