Recombinant Human CD19 (C-Fc) (C572)
产品说明(Description)
Recombinant Human CD19 is produced by our Mammalian expression system and the target gene encoding Pro20-Lys291 is expressed with a Fc tag at the C-terminus.
Accession #: P15391
Known as: B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE and SEC-HPLC.
Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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SDS-PAGE |
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SEC-HPLC |
Bioactivity-Elisa:
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Immobilized Human FMC63 at 2μg/ml (100 μl/well) can bind Human CD19-Fc (Cat#C572).The ED50 of Recombinant Human CD19-Fc (Cat#C572) is 55.28 ng/ml. |
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Immobilized Human FMC63 at 2μg/ml (100 μl/well) can bind Human CD19-Fc (Cat#C572).The ED50 of Recombinant Human CD19-Fc (Cat#C572) is 55.28 ng/ml. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.
电泳(SDS-PAGE)
参考文献(Reference)
TRIFUNCTIONAL MOLECULE AND APPLICATION THEREOF[J]. Shuai CHEN. et al. 2020.
FOR RESEARCH USE ONLY