Recombinant Human Beta -Actin

Recombinant Human Beta -Actin (C-6His)  (CE15)

产品说明(Description)

Recombinant Human beta-Actin is produced by our E.coli expression system and the target gene encoding Asp2-Phe375 is expressed with a 6His tag at the C-terminus. 

Accession #: P60709

Known as: Actin Cytoplasmic 1; Beta-Actin; ACTB

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 10mM Tris-HCl, 2mMDTT, 10% Glycerol, pH 8.0.

 

质量控制(Quality Control)

 

Purity:            Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景(Background)

Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Knockout of ACTB and ACTG1 with CRISPR/Cas9(D10A) Technique Shows that Non-Muscle β and γ Actin Are Not Equal in Relation to Human Melanoma Cells’ Motility and Focal Adhesion Formation[J]. Natalia Malek. et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020.

 

FOR RESEARCH USE ONLY