Recombinant Mouse IL-1F2/IL-1

Recombinant Mouse IL-1F2/IL-1 beta  (C042)

 

产品说明(Description)

Recombinant Mouse Interleukin-1 beta is produced by our E.coli expression system and the target gene encoding Val118-Ser269 is expressed. 

Accession #: P10749

Known as: Interleukin-1 Beta; IL-1 Beta; Il1b

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 50mM TrisHCl, 50mM NaCl, pH 8.0.

 

质量控制(Quality Control) 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1β, designated interleukin 1β-converting enzyme (ICE), has been described. Mature human and mouse IL-1β share approximately 75% amino acid sequence identity and human IL-1β has been found to be active on murine cell lines.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome[J]. Qiang Qin. et al. Cell Reports. 2021.

Podocytes present antigen to activate specific T cell immune responses in inflammatory renal disease[J]. Shan Li. et al. JOURNAL OF PATHOLOGY. 2020.

Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β[J]. Zhifei Zhou. et al. Journal of Cellular and Molecular Medicine.2020.

Immunotherapy of patient with hepatocellular carcinoma using cytotoxic T lymphocytes ex vivo activated with tumor antigen-pulsed dendritic cells[J]. Ying Wang. et al. Journal of Cancer. 2018.

Kartogenin mediates cartilage regeneration by stimulating the IL-6/Stat3-dependent proliferation of cartilage stem/progenitor cells[J]. Tao Liu. et al. Biochemical and Biophysical Research Communications. 2020.

 

 

FOR RESEARCH USE ONLY