Recombinant Human VEGFR1 (C-Fc

Recombinant Human VEGFR1 (C-Fc) (CJ93)

产品说明(Description)

Recombinant Human Vascular Endothelial Growth Factor Receptor 1 is produced by our Mammalian expression system and the target gene encoding Ser27-Asn756 is expressed with a Fc tag at the C-terminus. 

Accession #: P17948

Known as: Vascular endothelial growth factor receptor 1; VEGFR-1;Fms-like tyrosine kinase 1;FLT-1;Tyrosine-protein kinase FRT;Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.        

 

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Human VEGF165 (Cat:C083) at 2μg/ml (100 μl/well) can bind  Human VEGFR1/FLT1(Cat:CJ93), The ED50 of Human VEGFR1/FLT1 (Cat:CJ93) is  36.4 ng/ml.	000000	Loaded Human VEGFR1-Fc(Cat#CJ93) on Protein A Biosensor, can bind  Human VEGF165(Cat#C083) with an affinity constant of 0.29 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Human Vascular endothelial growth factor receptor 1(VEGFR-1, FLT-1) is a member of the the class III subfamily of receptor tyrosine kinases (RTKs) and Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 is widely expressed in human tissues including normal lung, placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cellsand peripheral blood monocytes. VEGFR-1 contains seven Ig-like C2-type domains and one protein kinase domain. VEGFR-1is an essential receptor tyrosine kinase and plays an important role in theregulation of VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. It is also mediators of neurotrophic activity and regulators of hematopoietic development.  VEGFR-1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF.It may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells and promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. VEGFR-1 can also promote PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro).

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Soluble Fms-Like Tyrosine Kinase-1 Alters Cellular Metabolism and Mitochondrial Bioenergetics in Preeclampsia[J]. Lissette C. Sánchez-Aranguren. et al. frontiers in psychology. 2018.

 

 

FOR RESEARCH USE ONLY