Recombinant Human BAFF (N-Fc)

Recombinant Human BAFF (N-Fc) (CU71)

产品说明(Description)

Recombinant Human TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala134-Leu285 is expressed with a Fc tag at the N-terminus. 

Accession #: Q9Y275

Known as: Tumor necrosis factor ligand superfamily member 13B;  B lymphocyte stimulator; BLyS;  B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-BLI:
Loaded Human BCMA-His(Cat#CC28) on HIS1K Biosensor, can bind  Human BAFF-Fc(Cat#CU71) with an affinity constant of <10-3 nM as determined in BLI assay.		Loaded Human BCMA-His(Cat#C01B) on HIS1K Biosensor, can bind  Human BAFF-Fc(Cat#CU71) with an affinity constant of 0.63 nM as determined in BLI assay.
Loaded Cynomolgus BCMA-His(Cat#C01D) on HIS1K Biosensor, can bind  Human BAFF-Fc(Cat#CU71) with an affinity constant of 0.07 nM as determined in BLI assay.		Loaded Human BCMA-mFc(Cat#CW13) on AMC Biosensor, can bind  Human BAFF-Fc(Cat#CU71) with an affinity constant of <10-3 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

TNFSF13B/TNFSF20 belongs to the tumor necrosis factor family. It abundantly is expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Isoform 3 acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain. Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY