Recombinant Mouse IL-18 Bindin

Recombinant Mouse IL-18 Binding Protein Isoform d/IL-18 BPd  (CM45)

 

产品说明(Description)

Recombinant Mouse Interleukin-18 Binding Protein Isoform d  is produced by our Mammalian expression system and the target gene encoding Thr29-Ala193 is expressed with a Fc tag at the C-terminus. 

Accession #: Q9Z0M9

Known as: Interleukin 18 Binding Protein Isoform d; IL-18 BPd;Interleukin-18-binding protein;Interferon gamma-inducing factor-binding protein;Igifbp

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Interleukin 18 binding protein (IL-18BP) is a physiological inhibitor that acts through binding to the receptor-binding site of IL-18. IL-18 stimulates INF-γ, which then stimulates 18BP production via NF-κB. The interaction between IL-18 and IL-18BP has a significant role in the inflammation process. IL-18 BPs have four isoforms, a, b, c and d, which are spliced by different ways. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain which is essential to the binding and neutralizing properties of the binding proteins. The IL-18 BP isoforms b and d lack a complete Ig domain. The expression of IL-18 and the IL-18BP are indentified in immune tissues such as the spleen, but also in nonimmune cells including keratinocytes.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

1、Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice. Hiramoto K. et al. Systems biology in reproductive medicine. 2017.

2、Ultraviolet B eye irradiation aggravates atopic dermatitis via ACTH and NLRP3 inflammasome in NC/Nga mice. Hiramoto K. et al. Photodermatology, Photoimmunology & Photomedicine. 2017.

 

FOR RESEARCH USE ONLY