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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
—20℃,12个月
- 保质期:
—20℃,12个月
- 英文名:
详见说明书
- 库存:
充足
- 供应商:
上海禾午生物科技有限公司
- CAS号:
无
- 规格:
1ml
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文献和实验RNAse A Treatment of Mouse Cells
I also agree that, the purity of the RNAse is crucial for the success of the experiment. SL-2 cells are grown on cover slips at a density of 7x106 cells/ml. All treatments with CSK buffer may be omitted. The permeabilization is performed
RNase Protection Assay--RNA酶保护
with DEPC treated Q, autoclave 1X Hybridization Mixture 8 ml formamide 1 ml DEPC treated Q 1 ml 10X Hybridization Buffer 2X RNase Buffer 20 mM Tris 7.5 2 ml 1M Tris 7.5 10 mM EDTA 2 ml 500 mM EDTA 8.0 0.6 M NaCl 12 ml 5 M NaCl up to 100 ml DEPC
transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32P]UTP 1 µl T7 RNA
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