O-GlcNAc Antibody 抗体,orb396424,Biorbyt产品图

O-GlcNAc Antibody 抗体,orb396424

,Biorbyt
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  • ¥7956
  • Biorbyt已认证
  • orb396424
  • 英国
  • 2026年07月24日
  • ELISA, ICC, IF, IHC, WB
  • Mouse
  • All
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体名:

      O-GlcNAc Antibody 抗体

    • 抗体英文名:

      O-GlcNAc Antibody

    • 靶点:

      O-GlcNAc

    • 浓度:

      1 mg/ml

    • 应用范围:

      ELISA, ICC, IF, IHC, WB

    • 宿主:

      Mouse

    • 适应物种:

      All

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb396424

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Monoclonal

    • 保存条件:

      详询

    • 亚型:

      IgM

    • 免疫原:

      详询

    • 规格:

      100 ug

    类别: Antibodies

    描述: Mouse Anti-O-GalNAc (β-O-Linked N-Acetylgalactosamine) Monoclonal IgM

    克隆号: 9H6

    别名: β-O-Linked N-Acetylglucosamine, O-Linked N-Acetylglucosamine, O-GlcNAc, Beta-O-Linked N-Acetylglucosamine, N-Acetylglucosamine, GlcNac, 2-(Acetylamino)-2-deoxy-D-glucose, N-acetyl-D-glucosamine, NAG, β-D-(Acetylamino)-2-deoxy-glucopyranose, beta-N-Acetylglucosamine, N-Acetylglucosamine-modified protein, β-N-Acetylglucosamine

    应用稀释比例: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC-P (1:25)

    应用说明: A 1:1000 dilution was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    免疫原: Synthetic β-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).

    防腐剂: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

    纯化方法: PEG Purified

    特异性: Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.

    研究领域: Epigenetics & Chromatin

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GlcNAc Antibody (D) Composite.

    产品细节图片2
    Western Blot analysis of GlcNAc-BSA Conjugate showing detection of 67 kDa O-GlcNAc protein using Mouse Anti-O-GlcNAc Monoclonal Antibody, Clone 9H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: GlcNAc-BSA. Lane 4: GalNAc-BSA. Lane 5: Galactose-BSA. Lane 6: Glucose-BSA. Lane 7: Citrulline-BSA. Load: 2.0 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GlcNAc Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

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    图标文献和实验
    相关实验
    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

    • Antibody cleanup

      seven times, or as often as necessary to decrease the background. Pellet cells. Save antibody supernatant, which is now diluted 1:5 from its initial concentration. These antibodies can be stored at 4o C for several weeks or at -70o C for longer periods

    • Antibody Conjugation Protocol

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

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