PeproTech重组人sTNF Receptor Type

Description:

TNFRI belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TNF-α or TNF-β to TNFRI initiates a signal transduction pathway that results in the activation of the transduction factor NF-κB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. Soluble TNF Receptor I (sTNFRI) is capable of inhibiting TNF-α and TNF-β activities by acting as a decoy receptor that serves as a sink for the TNF ligands. The human TNFRI gene encodes for a 455 amino acid type I transmembrane protein, which contains a 21 amino acid signal sequence, a 190 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 221 amino acid cytoplasmic domain. Recombinant Human sTNF Receptor Type I is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine-rich, ligand-binding portion of the extracellular domain of the TNFRI protein.
 

Manufactured using all Animal-Free reagents.
 

Source:E.coli
 

Synonyms:soluble Tumor Necrosis Factor Type I, TNFRSF1A, TNFAR, p60, p55, CD120a, TNFR1
 

AA Sequence:MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN
 

Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.
 

Biological Activity:Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The  ED₅₀  for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-α, is 0.05 µg/ml.
 

Country Of Origin: USA
 

Research Use Only: Not for use in diagnostic or therapeutic procedures.