Rat Primary Colonic Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Primary Colonic Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1×10^6 cells and is delivered frozen. Rat Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Rat Primary Colonic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications. AcceGen大鼠原代结肠上皮细胞是从1日龄实验室新生大鼠组织中分离得到的。大鼠原代结肠上皮细胞在涂有明胶包衣液的T25组织培养瓶中培养0.5小时,在AcceGen细胞培养液中培养3-5天。细胞从烧瓶中分离出来,然后立即冷冻保存在小瓶中。每个小瓶包含1×10^6细胞,并被冷冻运送。 大鼠原代结肠上皮细胞以E-cadherin或ZO-1抗体免疫荧光染色为特征。大鼠原发性结肠上皮细胞对细菌、酵母、真菌和支原体均为阴性。在AcceGen规定的细胞培养条件下,细胞可按1:2的分裂比例扩增3-7代。不建议重复冻融细胞 对上皮细胞培养进行的标准生化程序包括细胞对细胞粘附和迁移的测定,RT-PCR, Western blotting,免疫沉淀,免疫荧光染色或免疫荧光流式细胞术或生成所需的研究应用细胞衍生物。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.