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文献和实验E-Z 96® M13 Isolation Vacuum Manifold Protocol
75-150ul of preheated (60°C) Elution Buffer (10mM Tris-HCl, pH 8.5) into the center of the membrane to each well of the E-Z® 96 DNA Plate. Incubate for 10 minutes. Apply the vacuum for 5 minutes to elute DNA. This represents approximately 75-80
Extraction of RNA for Bacteria
. Transfer the flow-through lysate into a new 1.5ml tube. 4. Add 0.5 volume of absolute ethanol (96-100%, room temperature ) to the mixture and mix well by pipetting. 5. Add 0.5 volume (250ul or 350ul) of absolute ethanol (room temperature
Gene Knockout Techniques(1) The Knockout Process
all digestion plates and add 100 ul sterile deionized water to each well with a Robbins Hydra 96 (be sure to bleach and wash syringes between plates; the bleach solution is household bleach diluted 1:50, and the washes are done with sterile distilled water
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