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文献和实验protein, Drosophila Wnt8 (Dwnt8 or WntD), indicating that this protocol is likelyapplicable to all Wnts. Key words: Wnt , Wnt3A , b-catenin , Purification , Blue Sepharose , Immobilized metal affinitychromatography (IMAC) , Gel filtration , Heparin
Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study
function. SEVENS (http://sevens.cbrc.jp/ ) is an integrated database that includes GPCR genes that are identified with high accuracy (99.4% sensitivity and 96.6% specificity) from various types of genomes, by a pipeline that integrates such software
自然染色质免疫沉淀实验设计（Native Chromatin Immunoprecipitation (N-ChIP)）
of nucleidepending on cell line. 1.5.Centrifuge samples (10,000 g, 20 min, 4°C). 1.6.Resuspend nuclei pellet in 25% [w/v] sucrose/TBS at 4x106 nuclei / ml and underlay with 0.5 vol of 50% [w/v] sucrose /TBS; centrifuge the samples (14,000 g, 25 min, 4°C
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