Cardiff Protein G Sepharose HP

Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose

G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.

Purification of Antibodies Using Protein A-Sepharose and FPLC

at any one time. Since the antibody combining site is left free, protein A, when covalently coupled to stationary supports like agarose or Sepharose beads, provides an excellent reagent for isolating immune complexes or immunoglobulins from a crude solution

Detection of Protein‐Protein Interactions by Coprecipitation

Table of Contents Strategic Planning Basic Protocol 1: Coprecipitating Proteins with Protein A/G–Sepharose Alternate Protocol