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文献和实验°C for X min(1min/kb) Final extension 72°C for 7 min Final soak 4-10°C 6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling
A quick method to isolate plant
down the silica particles (glass milk),5 sec.,remove supernatant.(The DNA in the solution will now hopefully be bound to the silica particles). Wash the silica pellet with 800μl wash solution (from the GeneClean II kit). Repeat the wash
6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling program. 7) Simplified hot start: Start the PCR program. Once the thermal cycler has reached 94°C
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