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- 2025年07月10日
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
N 3M5, Canada 1 Corresponding author (jczp@sri.utoronto.ca ) INTRODUCTION Differentiation of mouse embryonic stem cells (ESCs) or hematopoietic stem cells (HSCs) from fetal liver or bone marrow into T-lymphocytes can be achieved in vitro
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
细胞类别MACS分选产品流式细胞仪抗体干祖细胞Anti-Sca-1 MicroBeadsAnti-Sca-1 Multisort KitCD117 MicroBeadsLineage Cell Depletion Kit T细胞及T细胞亚群CD90(Thy1.2) MicroBeadsPan T Cell Isolation KitCD4(L3T4) MicroBeadsCD4+ T Cell Isolation Kit CD4+CD25+ Regulatory T Cell
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