重组人白血病抑制因子（LIF）

产品名：重组人白血病抑制因子（LIF）
产品号：LT0151
 
产品信息：
白血病抑制因子（Leukaemia Inhibitor Factor, LIF）是典型的多功能生长因子，对于细胞生长、增殖与分化有着广泛的作用，与胚胎发育，神经发育和造血系统的发育有密切的联系。LIF是目前研究最多、应用最广的一种胚胎干细胞分化抑制因子，主要应用于胚胎干细胞的体外培养和研究。
胚胎干细胞（Embryonic Stem Cell, ESC）来源于体外受精后发育至囊胚期的内细胞团细胞，可在体外培养进行长期的自我更新，具有较高的端粒酶活性。胚胎干细胞具有多分化潜能，可以分化为三个不同胚层的细胞和生殖细胞。
LIF可与胚胎干细胞表面的LIFR/gp130受体结合，通过几个重要的信号通路起作用：
·LIF激活JAK-STAT3通路抑制ES细胞分化。
·LIF激活PI3K通路维持ES细胞的自我更新。
·LIF激活SHP-2-Ras-ERK通路。
 
别名：Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)
 
英文描述：
LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa protein containing 180 amino acids residues, including three disulfide bonds.
 
表达宿主来源:
E.coli
 
氨基酸序列:
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
 
保存条件：4℃保存6个月，-20℃保存一年或-80℃长期保存。
 
纯度&内毒素：
Greater than 95% by SDS-PAGE
gel and HPLC analyses.
Endotoxin Level<0.5EU/μg


The purity of rhLIF was determined by SDS-PAGE of reduced rhLIF and staining overnight with Coomassie Blue.

生物活性：
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.15ng/mL, corresponding to a specific activity of >=6.67×10^6 U/mg.