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- 库存:
充足
- 供应商:
peprotech
- 规格:
500ug
Murine Eotaxin-2 (CCL24) (500ug)



小鼠 CCL24(Eotaxin-2)重组蛋白
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表达系统:大肠杆菌(E. coli)
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氨基酸序列:VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV
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分子量:10.3 kDa
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类别:重组蛋白
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类型:蛋白质
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纯度:经 SDS‑PAGE 凝胶与 HPLC 检测,纯度 ≥ 98%
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内毒素浓度:<1 EU/μg
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活性:在 10–100 ng/ml 浓度范围内,通过对小鼠淋巴细胞的趋化作用测定其生物学活性。
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标记:未偶联(未标记)
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形态:冻干粉
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纯化状态:已纯化
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成分:不含防腐剂
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储存条件:-20 ℃
应用
- 免疫印迹(WB):稀释度依实验而定
- ELISA:稀释度依实验而定
- 功能实验:稀释度依实验而定
- 体外实验(IV):4 篇文献支持
- 其他 PubMed 文献:4 篇
产品特殊信息
- 重组小鼠 Eotaxin‑2 为 10.3 kDa 蛋白,含 93 个氨基酸残基。
- 本品常温运输。
- 具体储存、操作及复溶方法,请参见对应批号的分析证书(CoA)。
补充说明
- CCL24 = Eotaxin-2:嗜酸性粒细胞趋化蛋白 - 2
- 品牌:PeproTech®
- 产品货号:250-22-1MG
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Product Details |
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Size |
500 μg |
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Species |
Mouse |
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Published Species |
Mouse, Human |
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Expression system |
E. coli |
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Amino acid sequence |
VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV |
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Molecular weight |
10.3 kDa |
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Class |
Recombinant |
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Type |
Protein |
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Purity |
98% by SDS-PAGE gel and HPLC analyses. |
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Endotoxin concentration |
<1 EU/µg |
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Activity |
Determined by its ability to chemoattract murine lymphocytes using a concentration range of 10-100 ng/ml. |
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Conjugate |
Unconjugated |
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Form |
Lyophilized |
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Purification |
purified |
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Contains |
no preservative |
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Storage conditions |
-20°C |
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Applications |
Tested Dilution |
Publications |
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Western Blot (WB) |
Assay-dependent |
- |
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ELISA (ELISA) |
Assay-dependent |
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Functional Assay (Functional) |
Assay-dependent |
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In vitro Assay (IV) |
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4 Publications |
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Miscellaneous PubMed (Misc) |
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4 Publications |
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Product Specific Information
Recombinant Murine Eotaxin-2 is a 10.3 kDa protein containing 93 amino acid residues.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot- specific Certificate of Analysis
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Mouse CCL24 (Eotaxin-2) Protein (250-22-1MG) in Functional |
| Bioassay analysis of Mouse CCL24 (Eotaxin-2) Recombinant Protein, PeproTech® (Product # 250-22-1MG). |
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文献和实验[1] Karcz TP, Whitehead GS, Nakano K, et al. UDP-glucose and P2Y14 receptor amplify allergen-induced airway eosinophilia. J Clin Invest. 2021;131(7):e140709. doi:10.1172/JCI140709
[2] Felton JM, Bouffi C, Schwartz JT, et al. Aiolos regulates eosinophil migration into tissues. Mucosal Immunol. 2021;14(6):1271-1281. doi:10.1038/s41385-021-00416-4
[3] Yagawa Y, Robertson-Tessi M, Zhou SL, Anderson ARA, Mulé JJ, Mailloux AW. Systematic Screening of Chemokines to Identify Candidates to Model and Create Ectopic Lymph Node Structures for Cancer Immunotherapy. Sci Rep. 2017;7(1):15996. Published 2017 Nov 22. doi:10.1038/s41598-017-15924-2
[4] Bahar MW, Kenyon JC, Putz MM, et al. Structure and function of A41, a vaccinia virus chemokine binding protein. PLoS Pathog. 2008;4(1):e5. doi:10.1371/journal.ppat.0040005
[5] Karcz TP, Whitehead GS, Nakano K, et al. UDP-glucose and P2Y14 receptor amplify allergen-induced airway eosinophilia. J Clin Invest. 2021;131(7):e140709. doi:10.1172/JCI140709
[6] Patial S, Lewis BW, Vo T, et al. Myeloid-IL4Rα is an indispensable link in IL-33-ILCs-IL-13-IL4Rα axis of eosinophil recruitment in murine lungs. Sci Rep. 2021;11(1):15465. Published 2021 Jul 29. doi:10.1038/s41598-021-94843-9
[7] Singh K, Davies G, Alenazi Y, Eaton JRO, Kawamura A, Bhattacharya S. Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities. Sci Rep. 2017;7(1):4267. Published 2017 Jun 27. doi:10.1038/s41598-017-04378-1
[8] Pope SM, Zimmermann N, Stringer KF, Karow ML, Rothenberg ME. The eotaxin chemokines and CCR3 are fundamental regulators of allergen-induced pulmonary eosinophilia. J Immunol. 2005;175(8):5341-5350. doi:10.4049/jimmunol.175.8.5341
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