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- 文献和实验
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- 英文名:
Sodium 3-(4-hydroxy-3-methoxyphenyl)acrylate
- 库存:
14
- 供应商:
柯雷生物
- CAS号:
24276-84-4
- 规格:
0.98
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
If necessary, re-reduce histone octamers by incubating in 10 mM DTT for 30 minutes (see note 2 and comment 2); Dialyse histone octamer three times 2 hours or more against at least 100 times volume of 5mM sodium cacodylate pH 6.0, 2 M NaCl
3) (60 mM)MALDI matrix A Prepare a saturated solution of {alpha}-cyano-4-hydroxy-cinnamic acid in:Ethanol (45% [v/v])H2O (45% [v/v])10% formic acid (10% [v/v]).Store in the dark at room temperature.Nickel-nitrilotriacetic acid (Ni-NTA) resinPhosphoprotein
Assay of Deoxyhypusine Hydroxylase Activity
. This method does require specialized equipment. The second method is based on counting the total TCA soluble radioactivity after sodium periodate oxidation of the reaction mixture, since the radiolabeled 4-amino-2-hydroxy butyl moiety of the hypusine residue
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