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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
一年
- 英文名:
Casein Blocking Solution
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
100mL/盒
产品及特点
即用型(1% w/v)Hammarsten级酪蛋白用于封闭非特异性位点。A 型: 溶于TBS 的1% (w/v)Hammarsten 级酪蛋白,Kathon 防腐剂,pH7.4,B 型: 溶于PBS 的1% (w/v)Hammarsten 级酪蛋白,Kathon 防腐剂,pH7.4。可用于在使用牛奶封闭背景高时作为替代品使用。
酪蛋白封堵液-Casein Blocking Solution低温运输,-20℃保存,有效期一年。0.5M DTT 2ml
0.5M DTT 10ml
10% SDS 50ml
TEMED 5ml
1M Tris-HCl,pH6.8 50ml
1.5M Tris-HCl,pH8.8 100ml
Western转膜缓冲液 (20x) 100ml
Western一/二抗稀释液 100ml
Western封闭液(BSA型) 100ml
Western洗涤液 250ml
Western一抗二抗去除液 250ml
BCIP工作液 50mg/ml 100ul
NBT工作液 50mg/ml 100ul
10% Sodium Azide (叠氮钠) 100ml
Protease Inhibitor A(广谱性) 1ml
Protease Inhibitor B(适用于细菌) 1ml
Protease Inhibitor C(适用于哺乳动物) 1ml
Protease Inhibitor D(适用于真菌) 1ml
Protease Inhibitor E(适用于哺乳动物) 1ml
Phosphatase Inhibitor M(广谱性) 1ml
Lysozyme/溶菌酶(>20KU/mg)10mg/ml 1ml
Lysozyme/溶菌酶 50mg/ml 5ml
RNase A (10mg/ml) 1ml
RNase A (100mg/ml) 0.5ml
Proteinase K (20mg/ml) 0.2ml
Proteinase K (20mg/ml) 1ml
DEPC水(DNase、RNase free) 100ml
DEPC水(DNase、RNase free) 500ml
RNase Inhibitor 2000U
TE pH 8.0 100ml
3M NaAc pH5.2 100ml
1M MgCl2溶液 100ml
RNA上样缓冲液(6×) 120ul
DNA上样缓冲液(6×Ⅳ) 1ml
Zoman-Green (EB升级换代产品) 1ml
EB/溴化乙锭(10mg/ml) 1ml
TAE电泳缓冲液(50×) 100ml
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文献和实验Blocking cDNA arrays printed on amine slides
'ok'). ____9. Lift slide rack out of dish, pour in the blocking solution and quickly add slides. (Note: Slides should be lowered into solution quickly to reduce chance of smearing. Thought is that lowering slowly means DNA
Targeted Exon Sequencing by In‐Solution Hybrid Selection
a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in?solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibodies, Jackson Immunolabs
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