- ￥2180
- 上海
- 慢病毒主要用于难转染的细胞，稳定株的制备，神经系统的定位注射实验等。
- 2025年12月29日
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慢病毒包装
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文献和实验该产品被引用文献
[01] Cell.(IF=41.582).Xu M, Xu H H, Lin Y, et al. LECT2, a ligand for Tie1, plays a crucial role in liver fibrogenesis[J]. 2019, 178(6): 1478-1492. e20.[慢病毒,肝纤维化]
[02] Cell Metabolism.(IF=27.287).Lv H, Lv G, Chen C, et al. NAD+ metabolism maintains inducible PD-L1 expression to drive tumor immune evasion[J]. 2021, 33(1): 110-127. e5.[慢病毒过表达、干扰,肿瘤免疫,肝癌]
[03] Blood.(IF=22.113).Liu F, Gao Y, Xu B, et al. PEG10 amplification at 7q21. 3 potentiates large-cell transformation in cutaneous T-cell lymphoma: PEG10 amplification potentiates LCT in CTCL[J]. 2021.[慢病毒干扰,淋巴瘤]
[04] Nature cell biology.(IF=17.728).Shen S M, Zhang C, Ge M K, et al. PTENα and PTENβ promote carcinogenesis through WDR5 and H3K4 trimethylation[J]. 2019, 21(11): 1436-1448.[慢病毒过表达、Cas9/dCas9,甲基化,肝癌]
[05] Journal of hematology & oncology.(IF=17.388).Yang L, Chen Y, Liu N, et al. Low expression of TRAF3IP2-AS1 promotes progression of NONO-TFE3 translocation renal cell carcinoma by stimulating N 6-methyladenosine of PARP1 mRNA and downregulating PTEN[J]. 2021, 14(1): 1-20.[慢病毒干扰,肾细胞癌]
[06] Autophagy.(IF=16.016).Wang B , Yin X , Gan W , et al. PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma[J]. Autophagy.[慢病毒过表达,肾细胞癌]
[07] Cell Death & Differentiation.(IF=15.828).Ji J, Ding K, Luo T, et al. TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα[J]. 2021, 28(1): 367-381.[慢病毒 过表达、Cas9,胶质瘤]
[08] Cell Death & Differentiation.(IF=15.828).Hou Y, Zhang Q, Pang W, et al. YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect[J]. 2021: 1-20.[慢病毒过表达,肿瘤代谢,胰腺癌]
[09] Cell Death & Differentiation.(IF=15.828).Ji J, Ding K, Luo T, et al. TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα[J]. 2021, 28(1): 367-381.[慢病毒过表达、Cas9,信号通路,胶质瘤]
[10] Nature communications.(IF=14.919).Song H, Song J, Cheng M, et al. METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells[J]. 2021, 12(1): 1-15.[慢病毒干扰,肿瘤免疫（）NK细胞,肝癌]
[11] Nature communications.(IF=14.919).Liu J, Liu Z X, Wu Q N, et al. Long noncoding RNA AGPG regulates PFKFB3-mediated tumor glycolytic reprogramming[J]. 2020, 11(1): 1-16.[慢病毒 过表达 干扰, 糖酵解, 食道鳞状细胞癌]
[12] Nature communications.(IF=14.919).Ge M K, Zhang N, Xia L, et al. FBXO22 degrades nuclear PTEN to promote tumorigenesis[J]. 2020, 11(1): 1-14.[慢病毒Cas9,结直肠癌]
[13] Nature communications.(IF=14.919).Zhang M, Yang W, Wang P, et al. CCL7 recruits cDC1 to promote antitumor immunity and facilitate checkpoint immunotherapy to non-small cell lung cancer[J]. 2020, 11(1): 1-17.[,腺病毒-CRE]
[14] Nature communications.(IF=14.919).Yang Z, Xu G, Wang B, et al. USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade[J]. 2021, 12(1): 1-14.[慢病毒 过表达,腺病毒-cre PD-1耐药]
[15] Nature Communications.(IF=14.919).Huang X, Pan L, Zuo Z, et al. LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling[J]. 2021, 12(1): 1-16.[慢病毒Cas9,肿瘤代谢, 胰腺癌]
[16] Nature communications.(IF=14.919).Wang Z, Wang F, Zhong J, et al. Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors[J]. 2020, 11(1): 1-13.[慢病毒 过表达,血管生产]
[17] Theranostics.(IF=11.556).Chen Y, Pan C, Wang X, et al. Silencing of METTL3 effectively hinders invasion and metastasis of prostate cancer cells[J]. 2021, 11(16): 7640.[慢病毒干扰,前列腺癌]
[18] Clinical and Translational Medicine.(IF=11.492).Wang C, Yang Z, Xu E, et al. Apolipoprotein C‐II induces EMT to promote gastric cancer peritoneal metastasis via PI3K/AKT/mTOR pathway[J]. 2021, 11(8): e522.[慢病毒 过表达 干扰,胃癌]
[19] Molecular Therapy.(IF=11.454).Li R, Zeng L, Zhao H, et al. ATXN2-mediated translation of TNFR1 promotes esophageal squamous cell carcinoma via m6A-dependent manner[J]. 2022.[慢病毒 过表达,食管鳞状细胞癌]
[20] Journal of Experimental & Clinical Cancer Research.(IF=11.161).Song C, Zhou C. HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis[J]. 2021, 40(1): 1-16.[慢病毒干扰、过表达,胃癌]
[02] Cell Metabolism.(IF=27.287).Lv H, Lv G, Chen C, et al. NAD+ metabolism maintains inducible PD-L1 expression to drive tumor immune evasion[J]. 2021, 33(1): 110-127. e5.[慢病毒过表达、干扰,肿瘤免疫,肝癌]
[03] Blood.(IF=22.113).Liu F, Gao Y, Xu B, et al. PEG10 amplification at 7q21. 3 potentiates large-cell transformation in cutaneous T-cell lymphoma: PEG10 amplification potentiates LCT in CTCL[J]. 2021.[慢病毒干扰,淋巴瘤]
[04] Nature cell biology.(IF=17.728).Shen S M, Zhang C, Ge M K, et al. PTENα and PTENβ promote carcinogenesis through WDR5 and H3K4 trimethylation[J]. 2019, 21(11): 1436-1448.[慢病毒过表达、Cas9/dCas9,甲基化,肝癌]
[05] Journal of hematology & oncology.(IF=17.388).Yang L, Chen Y, Liu N, et al. Low expression of TRAF3IP2-AS1 promotes progression of NONO-TFE3 translocation renal cell carcinoma by stimulating N 6-methyladenosine of PARP1 mRNA and downregulating PTEN[J]. 2021, 14(1): 1-20.[慢病毒干扰,肾细胞癌]
[06] Autophagy.(IF=16.016).Wang B , Yin X , Gan W , et al. PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma[J]. Autophagy.[慢病毒过表达,肾细胞癌]
[07] Cell Death & Differentiation.(IF=15.828).Ji J, Ding K, Luo T, et al. TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα[J]. 2021, 28(1): 367-381.[慢病毒 过表达、Cas9,胶质瘤]
[08] Cell Death & Differentiation.(IF=15.828).Hou Y, Zhang Q, Pang W, et al. YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect[J]. 2021: 1-20.[慢病毒过表达,肿瘤代谢,胰腺癌]
[09] Cell Death & Differentiation.(IF=15.828).Ji J, Ding K, Luo T, et al. TRIM22 activates NF-κB signaling in glioblastoma by accelerating the degradation of IκBα[J]. 2021, 28(1): 367-381.[慢病毒过表达、Cas9,信号通路,胶质瘤]
[10] Nature communications.(IF=14.919).Song H, Song J, Cheng M, et al. METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells[J]. 2021, 12(1): 1-15.[慢病毒干扰,肿瘤免疫（）NK细胞,肝癌]
[11] Nature communications.(IF=14.919).Liu J, Liu Z X, Wu Q N, et al. Long noncoding RNA AGPG regulates PFKFB3-mediated tumor glycolytic reprogramming[J]. 2020, 11(1): 1-16.[慢病毒 过表达 干扰, 糖酵解, 食道鳞状细胞癌]
[12] Nature communications.(IF=14.919).Ge M K, Zhang N, Xia L, et al. FBXO22 degrades nuclear PTEN to promote tumorigenesis[J]. 2020, 11(1): 1-14.[慢病毒Cas9,结直肠癌]
[13] Nature communications.(IF=14.919).Zhang M, Yang W, Wang P, et al. CCL7 recruits cDC1 to promote antitumor immunity and facilitate checkpoint immunotherapy to non-small cell lung cancer[J]. 2020, 11(1): 1-17.[,腺病毒-CRE]
[14] Nature communications.(IF=14.919).Yang Z, Xu G, Wang B, et al. USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade[J]. 2021, 12(1): 1-14.[慢病毒 过表达,腺病毒-cre PD-1耐药]
[15] Nature Communications.(IF=14.919).Huang X, Pan L, Zuo Z, et al. LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling[J]. 2021, 12(1): 1-16.[慢病毒Cas9,肿瘤代谢, 胰腺癌]
[16] Nature communications.(IF=14.919).Wang Z, Wang F, Zhong J, et al. Using apelin-based synthetic Notch receptors to detect angiogenesis and treat solid tumors[J]. 2020, 11(1): 1-13.[慢病毒 过表达,血管生产]
[17] Theranostics.(IF=11.556).Chen Y, Pan C, Wang X, et al. Silencing of METTL3 effectively hinders invasion and metastasis of prostate cancer cells[J]. 2021, 11(16): 7640.[慢病毒干扰,前列腺癌]
[18] Clinical and Translational Medicine.(IF=11.492).Wang C, Yang Z, Xu E, et al. Apolipoprotein C‐II induces EMT to promote gastric cancer peritoneal metastasis via PI3K/AKT/mTOR pathway[J]. 2021, 11(8): e522.[慢病毒 过表达 干扰,胃癌]
[19] Molecular Therapy.(IF=11.454).Li R, Zeng L, Zhao H, et al. ATXN2-mediated translation of TNFR1 promotes esophageal squamous cell carcinoma via m6A-dependent manner[J]. 2022.[慢病毒 过表达,食管鳞状细胞癌]
[20] Journal of Experimental & Clinical Cancer Research.(IF=11.161).Song C, Zhou C. HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis[J]. 2021, 40(1): 1-16.[慢病毒干扰、过表达,胃癌]
相关实验
慢病毒包装实验
hetingcopy 大家好，我现在正在做慢病毒包装，用的是CLONTECK的包装系统，第一次感染293T细胞后，荧光很亮，48H后收集病毒上清，离心后冻于-80度，一天后感染靶细胞，什么都没有？请问大家是怎末做的？谢谢。 大鹏鸟 看到荧光并不代表包装成功,它代表的只是你的质粒转进去了。做慢病毒包装时必须要搭配好几个质粒的比例。转染之后要能看到细胞形态的病变。收集上清的时候要注意尽快分装保存。有可能的话最好把细胞也裂解一下
相关专题 慢病毒包装技术专题 慢病毒(Lentiviruses)属于逆转录病毒科。慢病毒核蛋白质前整合复合物具有噬核特性，病毒基因组运输至细胞核，从而使慢病毒可以感染和在非有丝分裂细胞中复制。这一特性使慢病毒成为基因治疗的转移载体 。 HIV (Human immunodeficiency virus) 、EIAV (Equine infectious anemia virus) 、 FIV (Feline
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慢病毒包装
￥2180
