- 询价
- PeproTech
- 120-14E
- 美国
- 2025年09月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
PeproTech(派普泰克)
- 库存：
大量
- 英文名：
Recombinant Human Activin A (E.coli)
| Synonyms : |
None |
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| Description : |
Activin A is a TGF-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin. Human Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues. |
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| Catalog #: |
120-14E |
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| Source : |
E.coli |
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| Purity : |
Greater than 97% by SDS-PAGE gel and HPLC analyses. |
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| Endotoxin Level : |
Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
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| Biological Activity : |
The ED 50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. |
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| AA Sequence : |
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS |
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| PubMed Link : |
Activin+AND+A+AND+human |
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| Country of Origin : |
USA |
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文献和实验E.coli genomic library construct 求教
） 5. ethanol 沉淀，electrotransformation, 结果得到的colony比较少，0.5ug 连接产物沉淀后, dissolve in 20ul ddH2O, 2ul + 50ul cell, spread on 8 plates, 总共只得到约1000个colonies（由于vector是单酶切，这里面还有载体自连产物，电转感受态细胞的转化效率很高，10的7次方－10的8次方），我的目的是希望尽可能cover E.coli 2kb-5kb之间的gene，所以希望能够得到5000－1w左右
Maxiprep of plasmid DNA from E.coli protocol
of 17200 Xg for 10 mins at room temperature , gently aspirate out the supernatant and discard it. Dry the pellet in air for 2 - 5 mins . Add 500 µl of TE buffer . Resuspend pellet by vortexing/by shaking vigorously. Add 500 µl of 5M LiCl
-500 ug/ml条件下,Trx-PrPC 27-30呈高表达,表达量接近50%.但AMP浓度加到500 ug/mlAMP时则会使细菌的生长速度下降.说明一定的选择压力可以提高质粒的稳定性从而提高融合蛋白表达量,200 ug/ml的AMP浓度为最适表达浓度. 不同IPTG浓度对融合蛋白Trx-PrPC 27-30的表达量无明显影响,0.1 mM的IPTG即可达到38%的表达量,1 mM IPTG可达最高表达量;乳糖诱导条件下融合蛋白Trx-PrPC 27-30的表达量随乳糖浓度增加而逐渐
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