血管紧张素Ⅱ1A型受体抗体

英文名称Angiotensin II type 1A receptor
中文名称血管紧张素Ⅱ1A型受体抗体
别 名AGTR1; Agtr1a; AT1; AT1A; AT1AR; Type 1 angiotensin II receptor; AGTR1_HUMAN; AGTR1B; AT2R1; AT2R1B.
说 明 书50ul 100ul 200ul
研究领域心血管 细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
血管紧张素Ⅱ1A型受体抗体产品应用WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3ug/Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量41kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from the middle of human Angiotensin II type 1A receptor:101-200/359 <Extracellular>
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The Angiotensin II type 1 receptor (AT1) is the primary effector of Angiotensin II, a key regulator of blood pressure and fluid homeostasis. It is involved in pathogenesis of several cardiovascular diseases such as hypertension, cardiac hypertrophy and congestive heart failure. Angiotensin II interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). AT1 has three isoforms in rodents: AT1A (359 aa), AT1B (359 aa), and AT1C (177 aa). Rat AT1's are predicted to contain seven transmembrane domains. The N-terminus is predicted to be extracellular, while the C-terminus is predicted to be cytoplasmic. AT1's are expressed in the liver, kidney, aorta, lung, uterus, ovary, spleen, heart, adrenal and vascular smooth muscle.

Function:
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit:
Interacts with MAS1 (Probable). Interacts with ARRB1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver, lung, adrenal and adrenocortical adenomas.

Post-translational modifications:
C-terminal Ser or Thr residues may be phosphorylated.

DISEASE:
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P30556

Gene ID:
185

Database links:
Entrez Gene: 185 Human
Entrez Gene: 24180 Rat
Entrez Gene: 81638 Rat
Omim: 106165 Human
SwissProt: P30556 Human
SwissProt: P34976 Rabbit
SwissProt: P25095 Rat
SwissProt: P29089 Rat
Unigene: 477887 Human
Unigene: 728754 Human


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


Sample:
Liver (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Adrenal gland (Rat) Lysate at 40 ug
Primary: Anti-Angiotensin II type 1A receptor (bs-2132R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 56 kD