- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0297
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pVL1392
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μg
pVL1392
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0297 | pVL1392 | 20μl |
¥请询价 |
使用说明
基本信息
| 启动子: | Polyhedrin |
|---|---|
| 复制子: | ColE1 ori |
| 终止子: | polyhedrin 3 |
| 质粒分类: | 昆虫细胞杆状病毒组成型表达质粒 |
| 质粒大小: | 9032bp |
| 原核抗性: | 氨苄青霉素Ampicillin |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 昆虫细胞 |
| 诱导方式: | 组成性表达，无需诱导 |
| 5'测序引物: | Polyhedrin-F：AAATGATAACCATCTCGC |
| 3'测序引物: | Polyhedrin-R：GTCCAAGTTTCCCTG |
质粒简介
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.
• Multiple cloning site polylinker, positioned in opposite orientations
• Recombination sequences for insertion into the baculovirus genome
• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein Features for growth and maintenance in E. coli:
• Ampicillin gene for selection
• pMB1 origin of replication from pUC19 for high copy number growth
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文献和实验vectors contain a strategically located short segment of DNA known as a polylinker that has been synthesized to contain a variety of restriction sites that are not present elsewhere in the plasmid. You can refer to your restriction map of the PVL1392
在目的基因起始密码子ATG前加上这一个序列 pVL1393， 昆虫表达载体 pIEX/Bac-1 昆虫表达载体 pTET on pTeT off pTER-tight pBAD HisA amp pBAD myc HisA amp pACYC184 amp pBluescript II SK (+) amp pIRESneo3 pPICZ α B pVL1393， pIEX/Bac-1 pcmv-myc P2
that has been synthesized to contain a variety of restriction sites that are not present elsewhere in the plasmid. You can refer to your restriction map of the PVL1392 vector to visualize this.The expression of a chimeric plasmid in a bacterial host was first demonstrated
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