- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0730
- 2025年07月13日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pLVTHM
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μg
pLVTHM
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0730 | pLVTHM | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | EF-1α ,H1,SV40 |
|---|---|
| 复制子: | pUC ori,SV40 ori |
| 终止子: | SV40 poly(A) signal，bGH poly(A) signal |
| 质粒分类: | 病毒系列，慢病毒干扰载体 |
| 质粒大小: | 11085bp |
| 原核抗性: | Amp |
| 克隆菌株: | Stbl3 |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | 根据序列设计引物 |
| 3'测序引物: | WPRE-R：CATAGCGTAAAAGGAGCAACA |
质粒简介
pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab. pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI. Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation . The plasmid needs to be grown in a Dam+ bacterial strain in order to use ClaI for cloning. Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work). Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid).
质粒图谱
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¥800 - 1380
