- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0183
- 2025年07月16日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pCEP4
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μl
pCEP4
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0183 | pCEP4 | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | CMV |
|---|---|
| 复制子: | pUC ori |
| 终止子: | SV40 poly(A) signal |
| 质粒大小: | 10186bp |
| 原核抗性: | 氨苄青霉素Ampicillin |
| 筛选标记: | 潮霉素Hygromycin |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 293T等哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
质粒简介
pCEP4 is an episomal mammalian expression vector that uses thecytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
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载体pCEP4
¥800 - 1380
