已经优化了编码野生型GFP的红移变体EGFP具有更亮的荧光,在哺乳动物细胞中表达较高(最大激发波长= 488nm,最大释放波长= 507nm)。 EGFP基因编码序列,含有190多个沉默碱基变化,符合人类密码子使用偏好。EGFP序列的一侧已经转化为KozaK的一致的翻译起始位点,这进一步提高了真核细胞中翻译的效率。
A red shift variant of the pEGFP-C2 encoding wild-type GFP has been optimized to have a brighter fluorescence, with higher expression in mammalian cells (maximum excitation wavelength =488nm, maximum release wavelength =507nm).
EGFP gene coding sequence, containing more than 190 silent base changes, which in line with the human codon usage preference.
One side of the EGFP sequence has been transformed into a consistent translation initiation site of KozaK, which further improves the efficiency of translation in eukaryotic cells.