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RT(常温）
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上海一基实业有限公司
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99590-86-0
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
to ~37℃ by soaking in warm water.If possible,keep a separate centrifuge and SS34 rotor at 4℃. 5.PEM buffer: 0.1 M PIPES,1.0 mM EGTA,0.5 mM MgCl2,pH 6.9,prepare 50 ml.Keep ~20 ml at 37℃ and the rest on ice. 6.DMSO. 7.TRSE,Molecular Probes C-1171.Prepare
the photolytic release of Ca2+ by flash photolysis of caged Ca2+ using compounds, such as o -nitrophenyl EGTA, introduced into the cell as the acetoxymethyl (AM) ester (NP-EGTA, AM). This method can be used in both intact and permeabilized cells.
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
to cross the plasma membrane was overcome by the addition of an acetoxymethyl ester group (AM), thus producing a lipophilic, membrane-permeant molecule (quin-2 AM) that, once within the cytoplasm, was subject to attack by intracellular enzymes
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