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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT(常温)
- 保质期:
详见标签
- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
443-72-1
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文献和实验Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents
, easy to reproduce, and that produces stable oligonucleotide arrays. Two new reagents, viz., [N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)- triethoxysilylpropyl-3-amine], and [N-(3-trifluoroethanesulfonyloxypropyl)anthraquinone-2- carboxamide
to replace combinattons of leucme coupled with valine in pepttde sequences. The preparation described is the final method developed, after many early less successful attempts. The synthesis mvolves (Fig. 1 ) the coupling of 1-bromo-5-methyl-2-hexene 3 and N
of 7‐bromo‐7‐deaza‐2′‐deoxyisoguanosine (S.4c ) is carried out by glycosylation of a brominated nucleobase followed by amination at C4 and selective deamination at C2′. NBS, N ‐bromosuccinimide; Piv, pivaloyl; TDA‐1, tris[2‐(2‐methoxyethoxy)ethyl]amine
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