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- Invitrogen
- V13245
- 2025年10月03日
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文献和实验. During apoptosis, PS is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. 实验步骤 Example
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
High‐Throughput Cytotoxicity Screening by Propidium Iodide Staining
Figure 9.24.1 Flow cytometry analysis parameters for cytotoxicity assay. Plots of forward scatter (Lin FS) versus red propidium iodide (PI) cell fluorescence (Log PI) allow distinction of viable cells (rectangular gate labeled “live”) and dead cells
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