- ¥1000
- HZbscienc
- 中国/欧洲/美国
- HZ0078
- 2025年07月14日
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- 技术资料
- 保存条件：
-20摄氏度保存
- 保质期：
1年
- 英文名：
pDsRED-Monomer-N1
- 库存：
大量
- 供应商：
上海沪震生物科技有限公司
- 规格：
盒
pDsRED-Monomer-N1产品信息
订购信息
| 货号 | 载体名称 | 出品公司 | 载体用途 | 价格 | 到货周期 |
| HZ0078 | pDsRED-Monomer-N1 | 荧光蛋白报告载体 | 1000 | 现货 |
产品参数
5' Sequencing 1 Primer: CMV-F, DsRed1-N
Selectable Marker: Neomycin
载体描述
pDsRed-Monomer-N1 is a mammalian expression vector that encodes DsRed-Monomer (DsRed.M1), a monomeric mutant derived from the tetrameric Discosoma sp. red fluorescent protein DsRed. DsRed-Monomer contains forty-five amino acid substitutions. When DsRed-Monomer is expressed in mammalian cell cultures, red fluorescent cells can be detected by either fluorescence microscopy or flow cytometry 12–16 hr after transfection (DsRed-Monomer excitation and emission maxima = 557 nm and 592 nm, respectively). The DsRed-Monomer coding sequence is human codon-optimized for high expression in mammalian cells. DsRed-Monomer is well suited for use as a fusion tag. The multiple cloning site (MCS) in pDsRed-Monomer-N1 is positioned between the immediate early promoter of CMV (PCMV IE) and the DsRed-Monomer coding sequence. Genes cloned into the MCS are expressed as fusions to the N-terminus of DsRed-Monomer if they are in the same reading frame as DsRed-Monomer and there are no intervening stop codons. A Kozak consensus sequence is located immediately upstream of the DsRed-Monomer gene to enhance translational efficiency in eukaryotic systems. SV40 polyadenylation signals downstream of the DsRed-Monomer gene direct proper processing of the 3' end of the DsRed-Monomer mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neor) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of the cassette confers kanamycin resistance in E. coli.
载体序列
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文献和实验啦之类的那一块。这家伙是得了炸药奖的。Clontech公司构建了大量荧光定位质粒，如pEGFP-N1，pDsRed2-N1，pDsRed-Monomer-C1等等。 萤光，英文luminescence，即发光或称生物发光，是指萤火虫荧光素酶，海肾荧光素酶等在底物存在（实际上还需要ATP,氧，辅酶A等，这些试剂盒中已经加入）下发出的光，是生物化学反应，他的实质是酶，没有底物是不能发光的。把LUC利用的最好的是PROMEGA公司，他们有大量试剂盒是基于这两种荧光素酶的，最常见的是检测启动子或增强子活性
?pDsRed2-N1(DsRed1-N-f)DsRed1-C-rpDsRed2-C1(DsRed1-N-f)DsRed1-C-rpDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3’pDsRed-ExpressdsRed1_N_primer/CMV-FdsRed1_C_primer/CMV-RpDSRED-monomer-N1(KAN+)dsRed1_N_primer pDSRED-N1(KAN+)pEGFP-N-5’pDSRED-N-RpEASY-BluntM13FT7/ M13
Y1Hgold PEGFP-1 PEGFP-C1 PEGFP-C2 PEGFP-C3 PEGFP-N1 PDsred2-ER PDsred2-N1 PDsred1-C1
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