MFN2 Antibody抗体，orb393107，bior

价格￥1560

品牌biorbyt
货号orb393107
产地英国
更新日期2025年12月04日

应用范围IF, IHC, WB
宿主Rabbit
适应物种Human, Monkey

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抗体名：
MFN2 Antibody抗体
抗体英文名：
MFN2 Antibody
应用范围：
IF, IHC, WB
宿主：
Rabbit
适应物种：
Human, Monkey
保质期：
6-12个月
抗原来源：
详询
目录编号：
orb393107
级别：
科研级
库存：
88
供应商：
biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MFN2. The exact sequence is proprietary.
规格：
30 ul

产品描述：The MFN2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MFN2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

别名:CPRP1; KIAA0214; Mitofusin-2; Transmembrane GTPase MFN2

免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MFN2. The exact sequence is proprietary.

应用稀释比例：WB: 1:500:1000, IHC-P: 1:100:200

纯化:The antibody was purified by immunogen affinity chromatography.

保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Entrez:170731, 9927

UniProt ID:O95140, Q80U63, Q8R500

Note:For research use only.

Western blot analysis of MFN2 expression in HepG2 (A), HuvEc (B) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 86 kD)

Immunohistochemical analysis of MFN2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MFN2 staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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【原创】请教高手，我的这种设计不同种属的同源基因引物的方法是否正确？
开设计，既麻烦而且贵，能否一个引物就搞定了哪？因为我最想要大鼠的，所以我就首先查大鼠mitofusin2基因的信息：在NCBI entrez gene的页面下输入：mitofusin2 http://www.ncbi.nlm.nih.gov/sites/entrez 出来很多基因信息，我选择第12的一条，是Rattus norvegicus(大鼠的），然后点开。在新页面中查看到 Homology 一项： Homologs of the Mfn2 gene The Mfn2 gene
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
sapiens replication protein A2, 32kDa (RPA2), mRNA 537 NM_004356 Homo sapiens CD81 antigen (target of antiproliferative antibody 1) (CD81), mRNA 3638 NM_001743 Homo sapiens calmodulin 2 (phosphorylase kinase, delta) (CALM2), mRNA 4675 NM_004231
所有的看家基因(housekeeping genes)的列表+引物设计服务
Homo sapiens mitofusin 2 (MFN2), mRNA 453 NM_006796 Homo sapiens AFG3 ATPase family gene 3-like 2 (yeast) (AFG3L2), nuclear gene encoding mitochondrial protein, mRNA 724 NM_006666

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MFN2 Antibody抗体，orb393107，biorbyt

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