- ¥1250
- 上海雅吉生物科技有限公司
- YSm-4577M
- 2025年07月16日
- ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-2732 （石蜡切片需做抗原修复） not yet tested in other applications.
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
FITC
- 宿主：
Rabbit
- 应用范围：
ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-2732 （石蜡切片需做抗原修复） not yet tested in other applications.
- 抗体英文名：
LAMR1(M1S3)
- 规格：
0.1ml 0.2234ml
上海雅吉生物科技有限公司专注于抗体研发生产十五年，拥有专业的抗体研发生产团队，可为客户提供包含抗原设计—多肽合成、蛋白表达—动物免疫—抗体纯化—抗体标记—免疫学检测等项目的一站式抗体制备服务。根据客户的实验需求，现提供兔、小鼠、大鼠、羊、狗等多种动物种属的多克隆抗体制备服务。
英文名称LAMR1(5D4)
中文名称层粘连蛋白受体1单克隆抗体
别 名37LRP; P37-kDa laminin receptor precursor; MGr1-Ag; laminin receptor 1; 67kD, ribosomal protein SA; 34/67 kDa laminin receptor; 40S ribosomal protein SA; 67LR; Colon carcinoma laminin-binding protein; LAMBR; Laminin Receptor 1; LAMR 1; LAMR1; LRP; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; RPSA; 67kD laminin receptor.
规格价格ul 100ul
文献引用：
pecific References (1) | bsm-0940M has been referenced in 1 publications.[IF=2.40] Wang, Leilei, et al. "67‐kDa Laminin Receptor (LR1) Contributes to Hypoxia‐induced Migration and Invasion of Trophoblast‐like Cells by Mediating Matrix Metalloproteinase (MMP)‐9." Clinical and Experimental Pharmacology and Physiology (2015). WB ; Human.
研究领域肿瘤 细胞凋亡 细胞膜受体
抗体来源Mouse
克隆类型Monoclonal
克 隆 号5D4
交叉反应 Human, Mouse, Rat, Dog,
层粘连蛋白受体1单克隆抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32.7kDa
细胞定位细胞核 细胞浆 细胞膜 细胞外基质
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from midlle of human LAMR1:2-100/295
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Function:
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
Subunit:
Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK. Interacts with PRNP. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.
Subcellular Location:
Cell membrane. Cytoplasm. Nucleus. Note=67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus. Co-localizes with PPP1R16B in the cell membrane.
Post-translational modifications:
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
Similarity:
Belongs to the ribosomal protein S2P family.
SWISS:
P08865
Gene ID:
3921
Database links:
Entrez Gene: 3921 Human
Entrez Gene: 100505031 Mouse
Entrez Gene: 16785 Mouse
Entrez Gene: 29236 Rat
Omim: 150370 Human
SwissProt: P08865 Human
SwissProt: P14206 Mouse
SwissProt: P38983 Rat
Unigene: 449909 Human
Unigene: 311972 Mouse
Unigene: 391708 Mouse
Unigene: 4071 Mouse
Unigene: 121972 Rat
Unigene: 161973 Rat
Unigene: 999 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
层粘连蛋白受体1单克隆抗体层粘连蛋白受体1（37LRP）是层粘连蛋白受体重要的前体，是近期发现的-胃癌耐药相关蛋白。
【抗体的鉴定】
筛选杂交瘤细胞通过选择性培养而获得杂交细胞系中，仅少数能分泌针对免疫原的特异性抗体。一般在杂交瘤细胞布满孔底1／10面积时，即可开始检测特异性抗体，筛选出所需要的杂交瘤细胞系。
检测抗体的方法应根据抗原的性质、抗体的类型不同，选择不同的筛选方法，一般以快速、简便、特异、敏感的方法为原则。
【抗体溶解方法】
方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或庆大霉素），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，最好分装后使用，避免反复冻融，可保证至少1年有效；
方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）
【抗体修复方法】
方法1：沸水浴修复，将盛有修复液和玻片的烧杯置于沸水浴环境，保持外部沸腾状态15min，自然冷却至室温；
方法2：微波修复，将盛有修复液和玻片的烧杯置于微波炉中，高火5min，停火3min，中火5min，自然冷却至室温。
DAB溶液配制：（组化及Western blot 显色）
称DAB 30mg，用0.05mol/L pH7.6 Trs-HCL缓冲液100ml溶解，如有沉淀，过滤后使用，一般新鲜配制使用；
使用前加入1% H2O2 1ml至1.5ml，H2O2浓度为0.01%，混均后使用。7、3′，3′，5′，5′，-四甲基联苯胺（TMB）
【溶液配制】
用二甲基甲酰胺（dimethylformamide，DMF）或无水乙醇，将TMB配成1%浓度，4°C保存半年以内使用；
使用前，用pH5.0磷酸-柠檬酸底物液9.9ml加入0.1ml（1mg/TMB）1% TMB溶液中，再按每毫升TMB加入30% H2O2 1ul，混匀后立即使用。2N H2SO4终止后，450nm测定OD值。
8、1%离子琼脂胶配制液
作免疫双相扩散，单相扩散及免疫电泳中使用。
英文名称LAMR1(5D4)
中文名称层粘连蛋白受体1单克隆抗体
别 名37LRP; P37-kDa laminin receptor precursor; MGr1-Ag; laminin receptor 1; 67kD, ribosomal protein SA; 34/67 kDa laminin receptor; 40S ribosomal protein SA; 67LR; Colon carcinoma laminin-binding protein; LAMBR; Laminin Receptor 1; LAMR 1; LAMR1; LRP; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; RPSA; 67kD laminin receptor.
规格价格ul 100ul
文献引用：
pecific References (1) | bsm-0940M has been referenced in 1 publications.[IF=2.40] Wang, Leilei, et al. "67‐kDa Laminin Receptor (LR1) Contributes to Hypoxia‐induced Migration and Invasion of Trophoblast‐like Cells by Mediating Matrix Metalloproteinase (MMP)‐9." Clinical and Experimental Pharmacology and Physiology (2015). WB ; Human.
研究领域肿瘤 细胞凋亡 细胞膜受体
抗体来源Mouse
克隆类型Monoclonal
克 隆 号5D4
交叉反应 Human, Mouse, Rat, Dog,
层粘连蛋白受体1单克隆抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量32.7kDa
细胞定位细胞核 细胞浆 细胞膜 细胞外基质
性 状Lyophilized or Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from midlle of human LAMR1:2-100/295
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Function:
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
Subunit:
Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK. Interacts with PRNP. The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.
Subcellular Location:
Cell membrane. Cytoplasm. Nucleus. Note=67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus. Co-localizes with PPP1R16B in the cell membrane.
Post-translational modifications:
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
Similarity:
Belongs to the ribosomal protein S2P family.
SWISS:
P08865
Gene ID:
3921
Database links:
Entrez Gene: 3921 Human
Entrez Gene: 100505031 Mouse
Entrez Gene: 16785 Mouse
Entrez Gene: 29236 Rat
Omim: 150370 Human
SwissProt: P08865 Human
SwissProt: P14206 Mouse
SwissProt: P38983 Rat
Unigene: 449909 Human
Unigene: 311972 Mouse
Unigene: 391708 Mouse
Unigene: 4071 Mouse
Unigene: 121972 Rat
Unigene: 161973 Rat
Unigene: 999 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
层粘连蛋白受体1单克隆抗体层粘连蛋白受体1（37LRP）是层粘连蛋白受体重要的前体，是近期发现的-胃癌耐药相关蛋白。
【抗体的鉴定】
筛选杂交瘤细胞通过选择性培养而获得杂交细胞系中，仅少数能分泌针对免疫原的特异性抗体。一般在杂交瘤细胞布满孔底1／10面积时，即可开始检测特异性抗体，筛选出所需要的杂交瘤细胞系。
检测抗体的方法应根据抗原的性质、抗体的类型不同，选择不同的筛选方法，一般以快速、简便、特异、敏感的方法为原则。
【抗体溶解方法】
方法1：我们的抗体（冻干粉）已经包含了0.01M PBS、1% BSA和0.1% 防腐剂（叠氮钠或庆大霉素），您只需要加入灭菌的双蒸水就行了。抗体溶解后，置于-20℃保存，最好分装后使用，避免反复冻融，可保证至少1年有效；
方法2：也可以只加入一半体积的双蒸水，再用一半体积的甘油补足，置于-20℃保存，这样抗体不会冻，有利于抗体的稳定。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
1) Reconstituting by directly adding 100uL of sterile, distilled water.
2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.
修复液：0.01M 枸橼酸（pH6.0）或者0.05M EDTA（pH8.0）
【抗体修复方法】
方法1：沸水浴修复，将盛有修复液和玻片的烧杯置于沸水浴环境，保持外部沸腾状态15min，自然冷却至室温；
方法2：微波修复，将盛有修复液和玻片的烧杯置于微波炉中，高火5min，停火3min，中火5min，自然冷却至室温。
DAB溶液配制：（组化及Western blot 显色）
称DAB 30mg，用0.05mol/L pH7.6 Trs-HCL缓冲液100ml溶解，如有沉淀，过滤后使用，一般新鲜配制使用；
使用前加入1% H2O2 1ml至1.5ml，H2O2浓度为0.01%，混均后使用。7、3′，3′，5′，5′，-四甲基联苯胺（TMB）
【溶液配制】
用二甲基甲酰胺（dimethylformamide，DMF）或无水乙醇，将TMB配成1%浓度，4°C保存半年以内使用；
使用前，用pH5.0磷酸-柠檬酸底物液9.9ml加入0.1ml（1mg/TMB）1% TMB溶液中，再按每毫升TMB加入30% H2O2 1ul，混匀后立即使用。2N H2SO4终止后，450nm测定OD值。
8、1%离子琼脂胶配制液
作免疫双相扩散，单相扩散及免疫电泳中使用。
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批次间稳定一致 重组抗体源于一段只编码一种重链和轻链的DNA序列，从而确保抗体的超高纯度。标准化的培养条件下，由哺乳动物细胞表达，保证了抗体质量的批次间稳定性。 图1：对抗体进行质谱分析，数据表明：REAfinity抗体纯度非常高，而杂交瘤来源的抗体是一种混合物。(A)对两个杂交瘤来源的单克隆抗体样本进行质谱分析，可以看出，它们都含有一条分子量接近23,635Da的污染轻链。(B)两个REAfinity抗体样本的质谱分析结果显示，其含有极高纯度的轻链。 通用型同型
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层粘连蛋白受体1单克隆抗体
¥1250
