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文献和实验that these four collections will each have gaps, due to (a) some genes being essential, (b) failure to obtain transformants that passed all 5 PCR confirmation tests. Central Storage: We will be storing 4 sets of A isolates in 2-ml tubes. For essential genes
that these four collections will each have gaps, due to (a) some genes being essential, (b) failure to obtain transformants that passed all 5 PCR confirmation tests. Central Storage: We will be storing 4 sets of A isolates in 2-ml tubes. For essential genes
Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be on it. Add 30 μl 95% EtOH/0.12 M NaOAc
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