Recombinant Human Chromodomain

Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein

to avoid the time and expense of human tissue culture. This chapter focuses on the subcloning, purification, and initial functional validation of the recombinant human mitochondrial single-stranded DNA-binding protein. The cDNA encoding the mature form

Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers

Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1 –3 ). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides

Isolation of Recombinant DNA Elongation Proteins

This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d